A Novel Purification Technology To Increase Processing Efficiency, Purity And Recovery Of Lentiviral Particles For Viral Vector Development

Immediate access to high binding surface area enables more effective processing of lentiviral vectors

Therapeutic developers face significant challenges in purifying cell and gene therapy modalities. Current processes rely on solutions developed for monoclonal antibodies and are not fit-for-purpose for viral vector purification. Resin chromatography has significant limitations, such as reduced yield, long processing time, and expense.

AstreAdept™ is a novel nanofiber material that addresses the challenges of purifying large and fragile modalities, which are often produced at low titer with high levels of contaminants.

AstreAdept™ has extremely accessible fibers which allows almost immediate access to high binding surface area at high flow rates. Here, we present how this technology has been incorporated into a spin column format, the Nereus LentiHERO™ to bring these advantages to viral vector purification at lab-scale.