A Gentler Workflow: Fc-fusion Protein Purification With Mixed-Mode And Anion Exchange Chromatography

Traditional Protein A affinity chromatography is the gold standard for Fc-fusion protein purification, but it can introduce challenges such as protein denaturation and degradation due to low pH elution. This webinar presents a proof-of-concept study demonstrating the use of a new weak anion exchange hydrophobic chromatography resin, Nuvia™ wPrime 2A, and a strong high-performance anion exchange resin, Nuvia HP-Q, for the purification of EGFR:Fc fusion proteins. The two-step process achieves high purity and yield, offering a gentler, non-affinity-based alternative that preserves protein integrity and is suitable when Protein A workflows are suboptimal. This webcast will provide insights into comparative performance, workflow flexibility, and practical considerations for implementing this approach in your process development.

Key Takeaways

• Mixed-mode and anion exchange chromatography can achieve comparable purity and yield to Protein A methods, offering more flexibility.
• Neutral pH purification reduces the risk of Fc-fusion protein denaturation and degradation.
• Effective for Fc-fusion proteins where affinity chromatography isn’t suitable, expanding options for biologics purification.