Downstream Processing White Papers and Case Studies
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Metal Removal From Fine Chemical Products
Metal contamination in various injectable or even oral medicines is a serious concern.
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Successfully Cultivate Hybridoma Cells In High Densities
4/18/2016
The Fibra-Cel packed-bed basket technology has been established as an excellent method for the growth of suspension and anchorage-dependent cell lines. By Ray Rose, Stacey Willard, and Ma Sha
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Comparison Of Five Cell Culture Media Demonstrating CHO Cell Culture Growth In Batch Or Fed-Batch Process For Commercial Operations
This case study details a two month, four phase project to design chemically-defined feed media that improved two simulated batch processes utilizing products by SAFC, Life Technologies, Thermo Fisher Scientific, and Irvine Scientific. By Mark C. Arjona, Evan R. Horowitz, Alex A. Jurisch, Jenny Y. Bang, Tom Fletcher, Irvine Scientific
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Automation Of A Homogeneous Proximity Assay For Detection Of Residual Protein A In Biological Therapeutics
Protein A is used in affinity chromatography for therapeutic antibody purification and has been shown to leach from columns during purification of antibodies resulting in contamination. Residual Protein A in therapeutic drugs could elicit mitogenic or immunological reactions in patients.
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Optimized Sterile Filtration of Pharmaceutical Solutions
11/12/2015
The cost of filtration and separation stages within the production of pharmaceuticals has been increasing along with the increased complexity of final drug products.
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Automating Protein Purification Processes Achieves Reliable Results And Reproducibility
10/16/2013
ÄKTA start is an easy-to-use, reliable protein purification system. In this application note it is described how Erica, a post-doctoral researcher, used ÄKTA start to purify GST-tagged protein using a predefined method template, a GSTrap FF column, and a Frac30 fraction collector.
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Chromatography Column Protection With 3M Purification LifeASSUREā¢ PLA Series Membrane Filters
9/14/2011
Protective, or guard, filters are often used in chromatography column purification steps to protect the column media from contaminants in the various fluids that come in contact with the column.
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Challenges And Solutions Of Scalable Cultivation For Anchorage Dependent Cells In Single-Use Bioreactor
Microcarrier (MC)-based suspension culture processes have been used to culture anchorage-dependent cells in bioreactors. However, scale-up of the process for high cell growth and productivity poses many challenges. By Brian Lee, Yas Hashimura, Daniel Giroux, Marcos Sousa, Joao Clemente, Manuel Carrondo, Siddharth Gupta, Michael McCaman, and Yong-Jae Kim
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White Paper: Economic Analysis Of Single-Use Tangential Flow Filtration For Biopharmaceutical Applications
6/10/2011
Prior to creating an accurate economic model for TFF, a thorough understanding of the process where the TFF is used is needed. A process model is a valuable tool when properly executed and it must accurately include all critical aspects of the purification process. By Michael LaBreck, Mark Perreault, Novasep
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Assessment Of New Developments In ADCC Assays
With many of the existing patents covering antibody therapies set to expire in the next few years, the development of biologic therapeutics similar to the original drug (biosimilars) has become increasingly important.