Downstream Processing White Papers and Case Studies
-
Maximizing Cell Growth And Isobutanol Production
4/18/2016
This application note describes the integration of a Thermo Scientific® Prima® dB Mass Spectrometer (MS) with DASGIP Parallel Bioreactor Control Systems implemented at Gevo®, Inc. in Englewood, Colorado. By James Glenn, Claudia M. Huether, and Christiane Schlottbom
-
Determination Of kLa Values Of Single-Use Bioreactors
8/3/2016
Because single use bioreactors offered by manufacturers vary in parameters critical for oxygen transfer, they are often not comparable to each other and to conventional glass and stainless steel vessels.
-
Cultivation Of CHO Suspension Cells And SEAP Expression
This application note describes the cultivation of CHO suspension cells in the wave-mixed Thermo Scientific HyPerforma Rocker Bioreactor with a maximum working volume of 5 L.
-
Metal Removal From Fine Chemical Products
Metal contamination in various injectable or even oral medicines is a serious concern.
-
Comparison Of Five Cell Culture Media Demonstrating CHO Cell Culture Growth In Batch Or Fed-Batch Process For Commercial Operations
This case study details a two month, four phase project to design chemically-defined feed media that improved two simulated batch processes utilizing products by SAFC, Life Technologies, Thermo Fisher Scientific, and Irvine Scientific. By Mark C. Arjona, Evan R. Horowitz, Alex A. Jurisch, Jenny Y. Bang, Tom Fletcher, Irvine Scientific
-
Gemin X Pharmaceuticals: Finding A Drug’s Mechanism Of Action
Gemin X, a pharmaceutical company focused on the discovery and development of advanced oncology
therapeutics, approached Metabolon to run a metabolism profile for one of their compounds—GMX1777. -
Improving DNA Removal from Bioprocess Purification Processes
Many new biological drug products produced using recombinant DNA technology, such as monoclonal antibodies, are produced in cell culture. Because therapeutic proteins such as monoclonal antibodies are produced in cell culture, impurities can result from the host cells, or cell substrates. By 3M Purification Inc.
-
Monoclonal Antibody Purification Platform Using High-Capacity Protein A And Mixed-Mode Chromatography
10/1/2016
The first step in purification of an important class of therapeutic proteins, the polyclonal or monoclonal antibodies (mAbs), is their capture from plasma or tissue culture supernatants. Protein A–based media are by far the most common class of affinity products used for this purpose.
-
A Purification Strategy For Clinical-Grade Monoclonal Antibody
10/3/2016
mAbs are currently the most important class of therapeutic proteins. Advances in upstream process technologies have led to tremendous improvement of mAb titers in mammalian cell culture.
-
Discussion Of Vial Closures And Stoppering Force In Pilot Freeze-Dryers
To answer the often asked question "Can my system stopper a full tray of the vials and stoppers I have selected?,", it is important to be able to recognize the force needed for your particular container-closure system and to compare that to the stoppering pressure specification provided by the freeze dryer manufacturer. By Leslie Mather