UPSTREAM PROCESSING FEATURED ARTICLES
How Computational Drug Discovery Is Disrupting Biopharma
NeuBase Therapeutics CEO Dr. Dietrich Stephan discusses the important role computational drug discovery plays as we emerge from what he characterizes as “a decades-long, multi-billion-dollar chemical engineering escapade with a very low success rate to find one drug.”
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![Cell]( "Beating Stem Cell Sourcing, Exosome Production Challenges")
Beating Stem Cell Sourcing, Exosome Production ChallengesUrgent medical needs like COVID-19 therapeutics are fueling new interest in cell-based therapies, despite a years-long, uphill social battle. Here’s how one company’s persistent, global pursuit of safely harvested biologics is positioning it to address the greatest medical need of our time.
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![20_01_BusOfBio_Logo_OL]( "Introducing The Business Of Biotech Podcast")
Introducing The Business Of Biotech PodcastBioProcess Online is proud to launch The Business Of Biotech, a podcast series dedicated to the leaders of new and emerging biopharma firms as a resource on their journey from drug discovery to success in the clinic. Here's a preview of our first season, stacked with insight from founders who have done just that.
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![bert_headshot]( "Abcentra Chief Looks Beyond The Clinical Horizon")
Abcentra Chief Looks Beyond The Clinical HorizonWhy Bert Liang, M.D., Ph.D. obsesses over at-scale manufacturing and regulatory challenges from the outset of clinical trials.
UPSTREAM PROCESSING WHITE PAPERS & CASE STUDIES
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![FiT_MaxiCaps-_20158]( "Accelerating Biopharmaceutical Development From DNA To Drug")
Accelerating Biopharmaceutical Development From DNA To DrugIn the last decade, single-use solutions for virtually every bioprocess unit have been developed, and considerable investments have been made by the industry into areas that underpin this technology.
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Live Cell Imaging Of GPCR Dependent Second-Messenger Systems
A significant proportion of academic and drug discovery research is devoted towards the elucidation of second messenger signaling cascades resulting from interactions between G-protein coupled receptors (GPCR) and their ligands. By Paul Held Ph. D. and Peter Banks, Ph. D., BioTek Instruments, Inc. and Paul Tewson, Anne Marie Quinn and Thom Hughes, Montana Molecular
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Semi-Automation Of A Non-Radioactive Antibody-Dependent Cell-Mediated Cytotoxicity (ADCC) Assay (Part I)
We describe the use of a non-radioactive, bioluminescent Antibody-Dependent Cell- Mediated Cytotoxicity (ADCC) assay. The protocol is a simple sequential addition of reagents and cells that can be readily automated with a simple, inexpensive pipetting station.
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Significantly Improve FoxP3 Staining In Human Whole Blood
Regulatory T-cells (Treg) play an essential role in the control of immune responses to self and foreign antigens maintaining the balance between immunity and tolerance.
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Better Characterization of Biomolecules Using Agilent AdvanceBio Reversed-Phase Columns
Reversed-phase (RP) is one of the three key techniques used in biochromatography and is particularly valuable because of its compatibility with LC/MS detection. And small particle improvements, such as those found in Agilent ZORBAX RRHD 300A, 1.8 um columns, make RP an attractive choice for many biopharmaceutical applications.
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Perfusion / Cell Retention: Attaching An ATF System To Different Bioreactors
There are three methods to attach an ATF™ System to a bioreactor, depending on the type of bioreactor you use.
UPSTREAM PROCESSING PRODUCTS
ÄKTA avant™ is a preparative chromatography system designed for fast and secure development of scalable methods and processes.
Mixed-mode chromatography resin is a top tier selection for the manipulation, purification and handling of molecules; providing an unmatched level of separation properties and resolution. The combination of cation exchange resin and metal affinity resin properties, allows multimodal chromatography to provide an access route for biomolecules to separate and the use of alternative chromatographic methods make these biomolecules seem homogenous.
The S.U.F. is engineered for optimal oxygen mass transfer, mixing, and temperature control, and performs comparably to traditional stainless steel systems. Using traditional, fundamental engineering principles, scale-up and process development work is streamlined – offering you cost savings and efficiency.
Macro-Prep hydrophobic interaction chromatography (HIC) media are methacrylate-based, 50 µm beads used for protein, polypeptide, enzyme, and nucleic acid purification.
Macro-Prep hydrophobic interaction chromatography (HIC) media are methacrylate-based, 50 µm beads used for protein, polypeptide, enzyme, and nucleic acid purification.
Resin are designed specifically for intermediate purification steps that remove host-cell contaminants from partially purified targets using strong or weak hydrophobic interactions. Methacrylate-based beads for protein and nucleic acid purification using hydrophobic interactions are available for purifying compounds that have weak or strong hydrophobic regions.
ReadyToProcess ULTA normal flow filter capsules are single-use filters designed for laboratory through process scale applications. Cytiva provides filtration solutions and support for integrated bioprocessing applications at every step and every scale of the drug development, validation and manufacturing process.
UPSTREAM PROCESSING MULTIMEDIA
Discover strategies for optimizing process control, refining media selection, and ensuring safety through rigorous contaminant detection to mitigate development risks.
Discover key strategies for scaling biologics from bench to pilot scale and learn how to overcome challenges in process development, including control of agitation, oxygen, CO₂, and nutrient supplementation.