Macro-Prep t-Butyl HIC Media

Macro-Prep hydrophobic interaction chromatography (HIC) media are methacrylate-based, 50 µm beads used for protein, polypeptide, enzyme, and nucleic acid purification. They are designed specifically for intermediate purification steps that remove host-cell contaminants from partially purified targets. Due to their rigidity and unique surface chemistry, these media are particularly suited for HIC operations requiring high throughput and high recovery of target.

These media are available in two functional forms:

• Macro-Prep methyl HIC support — weakly hydrophobic, for purifying compounds that have strong hydrophobic regions
• Macro-Prep t-butyl HIC support — mildly hydrophobic, for purifying compounds that have few or weakly hydrophobic regions

Both media are chemically and thermally stable. They are autoclavable and can withstand treatment in acid, chaotropic agents, or detergents while retaining high protein-binding capacities.

Macro-Prep t-butyl HIC support operates on a mechanism of interaction that is based on hydrophobicity and charge. The t-butyl groups are mildly hydrophobic. Depending on the pH of loading and elution buffers, the carboxyl groups can be exploited to ionically repel target molecules while the hydrophobic groups retain contaminants. This is an ideal strategy that minimizes the product loss commonly experienced with HIC media due to denaturation of proteins upon exposure to hydrophobic surfaces.

For more information, request bulletin 1841.

Regulatory support files are available upon request. Bio-Rad laboratories is an ISO 9001-registered corporation.