Cell Culture White Papers & Case Studies
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Fast Magnetic Bead Purification Of Interacting Cellular Proteins
12/14/2012
Biomedical research, and in particular cancer research, is progressing from focusing on small numbers of molecules or cellular events to global functional analysis.
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Why Magnetic Beads Have Several Advantages Over Direct Coupling
12/14/2012
The use of magnetic beads as the solid phase in ELISA (Enzyme-linked immunosorbent assay) has several advantages over direct coupling to the wells. These advantages include an increased available surface area and an even distribution of beads throughout the sample providing rapid and sensitive detection of low analyte concentrations (1. Liabakk et al).
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Meeting BioPharma Needs For Lower Operating Cost And Faster Time To Market
11/28/2012
Broad adoption awaits fully scalable GMP systems, improved and less costly disposables, better analytics, greater process automation and low-shear mixing.
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Automated Tissue Culture Cell Fixation And Staining In Microplates - Combination Washer Dispenser To Prepare Samples For Cellular Imaging
8/24/2012
Cellular imaging is becoming more important as a research tool in drug discovery and biomedical research. Low experimental sample numbers has historically allowed for manual implementation of the fixation and staining processes required prior to image analysis. By Paul Held PhD and Bridget Bishop, BioTek Instruments, Inc.
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Protein A Alternatives: Mixed Mode Resins For High Throughput mAb Feedstocks
8/16/2012
Mixed mode resins can serve as an alternative to protein A in intermediate processing. Implementation of mixed mode resins can reduce water required for filtration and time for column loading.
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Perfusion And Single-Use Bioreactors: A Partnership For Scale Up
11/3/2011
Perfusion has emerged as a viable technology for the commercial production of biopharmaceuticals. Perfusion solutions can offer very cost-efficient scale up solutions, driving down operating costs due to the smaller equipment and facilities needed as compared to batch or fed-batch systems.
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Chromatography Column Protection With 3M Purification LifeASSUREā¢ PLA Series Membrane Filters
9/14/2011
Protective, or guard, filters are often used in chromatography column purification steps to protect the column media from contaminants in the various fluids that come in contact with the column.
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Improving DNA Removal from Bioprocess Purification Processes
Many new biological drug products produced using recombinant DNA technology, such as monoclonal antibodies, are produced in cell culture. Because therapeutic proteins such as monoclonal antibodies are produced in cell culture, impurities can result from the host cells, or cell substrates. By 3M Purification Inc.
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Scale-Up Of Microbial Fermentation Using Recombinant E. Coli
Fermentation production facilities can utilize the Thermo Scientific™ HyPerforma™ Single-Use Fermentor (S.U.F.) instead of traditional stainless steel cleaning-in-place/ sterilization-in-place (CIP/SIP) fermentor vessels without modifying their existing procedures.
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Monitor The Effect Of Histone Methylase And Demethylase Inhibitors That Induce Apoptosis In Cancer Cell Lines
Histone lysine methylation is a reversible process, dynamically regulated by both lysine methyltransferases and demethylases. In general, methylation of histone H3 lysine 4 (H3K4me), H3K36, or H3K79 is associated with active transcription, whereas methylation of H3K9, H3K27, or H4K20 is associated with gene silencing.