Size exclusion chromatography (SEC) should separate proteins only according to their hydrodynamic radius (size). Using a design of experiments approach, this study describes the effect of buffer pH and NaCl concentration on proteins purified by SEC.
Size exclusion chromatography (SEC) is the go-to analytical method in the analysis of therapeutic protein drugs. This article describes the factors affecting the reproducibility of columns and how you can minimize column variation.
Size exclusion chromatography (SEC) is currently the most powerful chromatography technique for obtaining reliable information about the size of biomolecules under native conditions. This article presents fundamentals about analytical SEC and describes how new-generation agarose-based SEC columns are used in analysis of biomolecules.