With today’s strict time frames, the demands for automation and ease of use in bioprocessing are rapidly increasing. The ReadyToProcess WAVE 25 bioreactor system was developed with the goal of keeping the simplicity of single-use rocking bioreactor systems, while enhancing system features with time-saving automation technologies. This application note describes how ReadyToProcess WAVE 25 can help reduce hands-on time as much as 25% in fed-batch culturing, while delivering excellent culture performance. For an optimized perfusion application, the use of intelligent control software enabled accurate regulation of process parameters. Over 100 × 106 viable cells/mL was easily achieved in seven days.