Application Note

The Use Of Dynamic Control In Periodic Counter-Current Chromatography

Source: GE Healthcare Life Sciences

The ÄKTA™ pcc 75 chromatography system can be used for purification of target proteins in continuous downstream processes using periodic counter-current chromatography (PCC). PCC enables greater utilization of the chromatography medium (resin) capacity, allowing sample loading to much higher levels compared with what is possible in traditional batch chromatography.

This application note shows how the dynamic control functionality of ÄKTA pcc 75 can be used to adjust for variations in binding capacity of the chromatography medium or for changes in feed composition when, for example, perfusion cell culture is being employed. Dynamic control supports the process analytical technology (PAT) initiative defined by the U.S Food and Drug Administration (FDA).