By Joe S. Orlando, Ph.D., and Kimberly Mann
In biopharmaceutical manufacturing, the development of an expression system that stably produces high levels of desired recombinant protein can be a time-consuming and resource-intensive process. In brief, this process requires a vector containing the gene(s) of interest to be transfected into cells, and high-performing clones to be identified and isolated. This process takes many months, requiring large amounts of personnel time, supplies and equipment. Our goal in this study was to modify a well-defined cell line development system to generate high performing recombinants in a time- and resource-efficient manner.