Application Note

The Combination Of UCOE® Expression Technology With The CHOZN® Platform Reduces Resources And Cell Line Development Timelines

By Joe S. Orlando, Ph.D., and Kimberly Mann

scientist researching in laboratory pipetting into test tub

In biopharmaceutical manufacturing, the development of an expression system that stably produces high levels of desired recombinant protein can be a time-consuming and resource-intensive process. In brief, this process requires a vector containing the gene(s) of interest to be transfected into cells, and high-performing clones to be identified and isolated. This process takes many months, requiring large amounts of personnel time, supplies and equipment. Our goal in this study was to modify a well-defined cell line development system to generate high performing recombinants in a time- and resource-efficient manner. 

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