Skin Tissue - Apoptosis Detection Kits

The DermaTACS Kit was developed to provide researchers with an effective method for measuring apoptosis in skin samples. The kit was based on DNA end-labeling using termi-nal deoxynucleotidyl transferase (TdT) and modified nucleotides. Detection of incorporated molecules is achieved using a chromogenic substrate with a horseradish peroxidase detec-tion system. The extracellular scaffolding in skin tissue can make it problematic to perme-abilize and high background is a common problem with skin samples. The DermaTACS Kit includes two permeabilization reagents as options for permeabilization along with a detailed protocol with hints and tips for optimal labeling of skin samples based on empiri-cal testing. Low background is achieved using a combination of the high specificity of Trevigen's unique cation based optimized TdT labeling reaction using Apoptosis Grad TdT and TACS™ Blue Label as the visualization method. Also included is TACS-Nucleas to generate a positive control from your own sample. In conjunction with the excellent con-trast between the TACS Blue Label and the Nuclear Fast Red, the positive control ensures easy data interpretation.

This complete kit provides all the reagents required for labeling including two permeabiliza-tion reagents, labeling and stop buffers, labeling and detection reagents, and TACS-Nuclease.

Features:

• Fast. Requires less than 3 hours to complete.
• Exclusive, non-toxic TACS Safe TdT buffer - sodium cacodylate free.
• Unique buffer system produces more consistent labeling.
• Performance tested on skin derived samples.
• Includes exclusive Cytonin permeabilization reagent.
• Includes TACS-Nuclease solution for preparing sample-dependent positive controls.

Applications:

• In situdetection of apoptosis in fixed frozen, paraffin embedded, or plastic embedded cells and tissues.
• Assists in the identification of apoptotic morphologies.
• Helps resolve unique problems encountered when detecting apoptosis in skin sections.

Components:

• Labeling enzyme
• Chromogenic substrate
• Labeling buffer
• Optimized cation
• Counterstain
• Nucleotide mix
• Detection reagents
• TACS-Nuclease

Items not included: Reagents for dehydration and rehydration, H²O² , PBS buffer, and disposables.

Related Products:

Cell Proliferation Assays
DNA Laddering kits
Services and Assessories

Storage: Components are stored at -20°C, 4°C, and room temperature.

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