Application Note

Single-Use Workflow For Recovery Of A Domain Antibody From E. Coli Culture Feed In An Automated Manner

GE Heathcare

This application note demonstrates the performance of the automated ÄKTA™ readyflux single-use filtration system in microfiltration applications. Here, a domain antibody (dAb) expressed in E. coli was recovered from fermentation broth. The dAb-containing sample was clarified (2.5-times concentration) and further washed with three wash volumes, with a recovery of approximately 90% of product in the permeate.

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