Application Note

Screening, Ranking, And Epitope Mapping Of Anti-Human IL-9 Supernatants

Source: Bio-Rad Laboratories, Inc.

Monoclonal antibodies have become a particularly important resource for medical research, basic science, and disease diagnosis and therapy. Numerous applications utilizing monoclonal antibodies have been developed, including disease diagnosis, drug delivery to target cells (cancer treatment, immune suppression, HIV treatment), biosensors, organic molecule identification, blood clot detection, and septic shock treatment.

The ProteOn™ XPR36 protein interaction array system is a surface plasmon resonance (SPR) instrument with parallel sample processing capabilities that meets the challenge of high throughput while maintaining all the unique qualities of optical biosensors. The ProteOn system uses a novel microfluidics system to perform simultaneous measurements in an array of 36 interaction spots and 42 local reference spots (interspots). All stages of the experiment, from surface immobilization of “ligand” proteins to their subsequent reaction with “analyte” molecules, are measured in real time to provide comprehensive data for 36 interactions.