Screening And Optimization Of The Loading Conditions On Capto S

Increasing expression levels of recombinant proteins and the need for improved productivity and overall process economy puts extra demand on the next generation of chromatography media for the bio-pharmaceutical industry. A technique for significantly improving the capacity and mass transfer properties of ion exchange media is modification of the porous base matrix with a surface extender such as dextran. This has recently been used in the development of a new cation exchange medium, Capto™ S, and gives a substantial increase in binding capacity compared to the corresponding media without dextran.

Capto S is a chromatography medium based on the novel high flow agarose platform. The medium combines high rigidity with high dynamic binding capacity (DBC) and fast mass transfer to allow faster purification, more flexible process design and maximum cost efficiency. Previously launched products on this platform include Capto Q, Capto MMC and the MabSelect™ family. Capto S is a strong cation exchanger designed for capture and intermediate purification of recombinant proteins and as a second step in MAb purification.

To use the full potential of Capto S, we show how to thoroughly screen the loading parameters, conductivity and pH. In addition, recommended screening strategies are presented where a sequential approach and Design of Experiments are used and discussed.