Anti GFP, Monoclonal Antibodies

Green Fluorescent Protein (GFP) from jellyfish, Aequorea victria, has been used with increasing success as a marker protein for gene expression, intracellular processes and localization ranging from bacteria to transgenic mice. Wako offers mouse monoclonal antibodies applicable for various purposes.

Western Blotting: mFX75

Anti Green fluorescent Protein, Monoclonal Antibody (mFX75)

• Preparation - Prepared by a hybridoma, which is established by fusing mouse myeloma cells, NSO and spleen cells of BALB/c mice immunized with the the GFP’s partial peptide coupled with BSA after being immunized with BSA after being immunized with GFP. The hybridoma supernatant cultured by Hollow fiber instrument was purified by Protein A affinity chromatography.

Immunoprecipitation & Immunostaining: mFX73

Anti Green fluorescent Protein, Monoclonal Antibody (mFX73)

• Preparation - Prepared by a hybridoma, which is established by fusing mouse myeloma cells, NSO and spleen cells of BALB/c mice immunized with the GFP’s partial peptide coupled with BSA after being immunized with GFP. The hybridoma supernatant cultured by protein-free culture was subjected to ultrafiltration with an ultrafilter 30,000-molecular-weight cutoff.