Newsletter | December 2, 2022

12.02.22 -- Purification Of Viral Vectors And Vaccines
 
 

Large-scale downstream processing of viral vectors and vaccines can face a range of obstacles different from those of many biotherapeutics. These challenges mostly arise from the size and complexity of the vector, which can affect product purity and recovery. Improvements of purification tools are necessary to overcome these challenges and must be engineered for easy scalability to meet manufacturing demands. The following case studies examine the benefits of innovative resins and purification strategies.
Plasmid Purification
 
 

One of the technical challenges associated with producing plasmid DNA of gene therapy grade is the removal of contaminants. This case study demonstrates an effective purification of plasmid DNA from E. Coli.
Viral Vector Purification
 
 

Large-scale downstream processing of viruses can face challenges that mostly arise from the size and complexity of the virus. This can affect product purity and recovery. This webcast discusses improvements of purification tools to overcome these challenges.
A Two-Column Purification Strategy For Adenovirus
 
 

Adenovirus vectors are effective tools for the transfer of genetic material into mammalian cells. A two-column efficient purification process was developed for a recombinant adenovirus after screening several chromatography resins in this study.
Purification Development Of A Recombinant Retrovirus
 
 

This retrovirus purification case study presents data from the resin screening, endonuclease treatment and process scaling for the downstream processing of retrovirus-like particles intended for clinical trials.
Improving Efficiencies For H1N1 Virus Purification
 
 

This study explains the importance of resin screening and the scale-up purification of Influenza A H1N1 subtype in a single purification step.
CHT Prepacked Process-Scale Columns
 
 

Bio-Rad now offers validated, GMP ready-to-use CHT™ Ceramic Hydroxyapatite prepacked columns for all biopharmaceutical manufacturing applications. Foresight Pro Columns maintain performance after shipping and cycling. The prepacked columns have a standardized design and format and are available in a range of diameters and bed volumes.
Request To Speak To A Purification Scientist
 

The Bio‑Rad team of scientists is here to help accelerate your method and process development for biopharmaceutical manufacturing. We offer on-site and virtual meetings designed to meet your unique needs. With a wide range of chromatography resins, we can offer help and insight on a variety of topics. Learn more.
Resin Screening Tools
 

Screening chromatography resins can accelerate method development and optimize downstream purification workflows. Request resin samples.