Protein Absorbance Analyzer

Quantifying or identifying the absence or presence of proteins by directly measuring UV absorbance provides for fast, convenient results which are critical for biopharmaceutical and bioprocess applications. This method is widely used for detection of proteins during their separation by chromatography. Proteins exhibit a distinct UV absorption maximum at 280 nm, however nucleic acids sometimes present in the sample also absorb at 280 nm. The measurement at only 280 nm can identify the absence or presence of a protein and is more suited for the comparison of solutions of the same protein and less for absolute measurement. To make a more accurate determination of concentration, proteins can be monitored at 260 nm and 280 nm and the concentration can be calculated. CST offers photometers that monitor proteins exclusively at 280 nm or a dual wavelength photometer capable of monitoring at 260 nm and 280 nm simultaneously (other wavelengths available upon request). CST’s Protein Absorbance Analyzers are designed for continuous, realtime monitoring of protein.