Preparation and storage of the large number of buffers required for biopharmaceutical production can be a challenge, particularly in large production scale. In this application note, we show how in-line conditioning (IC) can help reduce the number of hold tanks and floor space needed for buffer preparation and storage. With IC, buffers of desired formulations are prepared from small-volume single-component stock solutions at the time of need. Formulating buffers from single-component stock solutions eliminates the need of conductivity and pH post-adjustments and adds the flexibility of on-demand production of different buffer formulations from the same stock solutions, simplifying buffer preparation. Compared with using manually formulated 1× buffers, our results show that the total tank volume could be reduced up to 90% and the footprint by 40% using IC.