Application Note

Media Adaptation For Suspension Cells

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The success of an experiment can rely heavily on the type of cell culture media used. Many choices are available and selecting the right media can ensure the optimal performance of your cells. In certain cases, you may need to adapt your current cell culture to a new medium. Transferring the cells directly into a new medium in one step may be possible, but a stepwise process may be necessary to minimize stress and to retain normal cellular phenotype. To adapt suspension CHO and 293 cultures, The Transfection Experts at Mirus Bio use the following procedure, which also serves as a general protocol that has been successful with multiple other cell types and media.

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