Maximize Monomer Recovery during mAb Purification

The Challenge
Is separating mAb monomers from aggregates a major challenge for you? Are traditional unimodal resins just not working? Despite using mixed-mode resins, are you unable to attain the expected yield while maintaining purity requirements?

Purification Tip
Each mixed-mode resin interacts in a unique way to the biomolecule you are purifying. Be sure to screen multiple mixed-mode resins to achieve the most efficient purification process optimized for highest possible target purity and recovery.

Our Approach
We compared the efficacy of three commercial mixed-mode resins for the purification of mAb S (approx. pI 6.9) in a Protein A eluate containing about 75% monomeric and 25% aggregated mAb. Each media reduced the aggregate levels to below 0.5% (Table 1). However, CHT Ceramic Hydroxyapatite Type I, 40 μm offered significantly higher monomer recovery in the smallest eluate volume (Figure 1 and Table 1). Refer to Bio-Rad bulletin 6749 for complete details on the study.