Application Note

Introduction To Pichia Pastoris In A Stirred-Tank Fermentor

Introduction To Pichia Pastoris In A Stirred-Tank Fermentor

By Richard Mirro, Eppendorf Inc.

This Applications Report presents a simple protocol for achieving high-density culture of Pichia pastoris (P. pastoris) cells using a New Brunswick benchtop, autoclavable stirred-tank fermentor or bioreactor.

Pichia pastoris is a methylotrophic yeast which provides a unique expression system for producing high levels of recombinant proteins, including enzymes, protease inhibitors, single-chain antibodies, and regulatory proteins at various levels. P. pastoris is an excellent culture for research and production because it offers a low-cost expression system similar to E. coli or other Prokaryotic cultures, combined with the post-translational modifications found in Eukaryotes.

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