Case Study

Intensified Seed Train: Driving Value Towards The Evolution Of Upstream Processes

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Perfusion involves the constant feeding of fresh media and removal of spent media and product while retaining high numbers of viable cells within the bioreactor vessel. With the increased pressure to drive down manufacturing costs and to improve facility flexibility, there is a need to implement new perfusion-based approaches. Ultra high-density cell culture processes such as concentrated fed-batch and steady-state perfusion allow for high density cell-cultures in both the seed train and production bioreactors. The use of perfusion bioreactors leads to a better control of process conditions and an increase in the quality of proteins.

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