For applications such as research studies and preclinical trials, quick production of small amounts of recombinant proteins is often required. Protein production in insect cells using the baculovirus expression vector system (BEVS) is commonly used for such applications, as insect cells are capable of most post-translational modifications required for a fully functional and soluble product and the genetic engineering steps are straightforward. In this application note, we describe how ReadyToProcess WAVE 25 bioreactor system can simplify operations in insect cell applications, while providing stable culture conditions though accurate process control. This single-use system is easy to use and can minimize hands-on time compared with culturing in shaker flasks. Further, the enhanced control capabilities allow for a repeatable process outcome with reliable data to support conclusions for future decision making.