To increase the yield of a target protein, fed-batch culture is often employed in biomanufacturing processes. This application note demonstrates the enhanced performance of commonly used cell lines for MAb production when supplemented with HyClone™ cell culture supplements. Cultures of Chinese hamster ovary (CHO) cells and the cholesterol-sensitive NS0 murine myeloma cell line were fed with HyClone Cell Boost™ nutrient supplements and HyClone LS1000 cholesterol supplement, respectively. Cell growth and MAb production in the fed-batch cultures were compared with batch cultures. The results demonstrate increased viable cell yields and productivity in the fed-batch cultures.
The fed-batch version of stirred-tank cell culture is commonly employed at large process scales. A primary driver of this mid-run addition of nutrients to a batch culture is to increase the quantity of product harvested. The prevalence of fed-batch over other modes is due to many practical factors such as reliability, scalability, and application breadth. Increases in the integral of viable cell concentration and volumetric productivity are examples of significant improvements commonly obtained.