Fast And Reproducible AAV2 Quantification With Automated ELISA

As part of the viral quantification process, researchers rely on various analytical methods to assess critical quality attributes. While traditional plate-based and dot blot assays can be useful, they are often complex, time-consuming, and non-standardized. To meet increased throughput and safety demands, fast and reproducible titration of viral particles is essential. This growing need has spurred development of more automated analytical solutions, which are paving the way for increases in facility throughput while helping to make quality control faster, more accurate, and more reproducible.

In this application note, we pair Simple Plex™ microfluidic cartridge technology on Ella™ with the PROGEN recombinant A20R antibody and demonstrate utility for accurate and efficient AAV2 capsid titer determination. We show how the superior specificity of the PROGEN A20 antibody helps ensure reliable AAV2 quantification. We also demonstrate that Simple Plex AAV2 viral titer assay data is highly correlated with data generated using the corresponding PROGEN AAV2 Titration ELISA 2.0R (PRAAV2R).