Application Note

Easy-to-use Alternative For Biomass Determination With A Soft Sensor

Source: Eppendorf, Inc

By Julian Kager, Jens Fricke, Ulrike Becken, and Christoph Herwig

Biomass concentration is a key process variable, which is used to identify trends and initiate process events in microbial bioprocesses. Real-time data on the biomass is essential to implement advanced control strategies, including the control of biomass-specific nutrient uptake rates.

Christoph Herwig’s group at Technische Universität Wien (TU Wien) has developed a biomass soft sensor based on readily available measurements and process parameters. They used the soft sensor to control various substrate uptake rates in parallel small-scale bioreactors.