Newsletter | April 7, 2021

04.07.21 -- Droplet Digital PCR Offers Precise And Consistent Results For AAV Assays

Transitioning Your Assay From Quantitative PCR To Droplet Digital PCR

There are similarities and differences between quantitative PCR (qPCR) and Droplet Digital PCR (ddPCR). This article shows data that provides guidance for migrating a qPCR assay that uses a standard curve to a ddPCR assay that directly quantifies a sequence without a standard curve. Learn about how transitioning your assay from quantitative PCR to droplet digital PCR can be simple and provide consistent results.

Viral Quantification: Adeno-Associated Virus Vector Genome Titer Assay

Recombinant adeno-associated virus (AAV) is rapidly becoming the vector of choice for human gene therapy applications due to the ability to achieve prolonged gene expression in a variety of tissues without a significant immune response or toxicity. Read about a highly precise method to determine AAV vector genome titers not unduly influenced by inefficient amplification or standard curve issues.

Droplet Digital PCR Applications Guide

Droplet digital polymerase chain reaction (ddPCR) was developed to provide high-precision, absolute quantification of nucleic acid target sequences with wide-ranging applications for both research and clinical diagnostic applications. ddPCR measures absolute quantities by counting nucleic acid molecules encapsulated in discrete, volumetrically defined water-in-oil droplet partitions.