Newsletter | November 18, 2021

11.18.21 -- Downstream Purification -- Vaccine And Therapeutics

 
 

Biotherapeutic purification processes involve multiple steps to isolate and purify the target molecule of interest. This typically consists of chromatography resins that must be carefully selected for each step in the workflow to meet the desired purification requirements of the molecule. The purification workflow will focus on process economics while maintaining high yield and purity of the final drug product. New chromatography resin designs provide a way to meet these demands.

Delivering Value For Biosimilar Purification
 
 

The global biosimilars market is set for continued growth. Biosimilars are significantly cheaper than biologics due to the shorter development time and fewer associated costs. Nonaffinity purification platforms are proposed to improve overall process economics and purification results.

 

Virus And Virus-Like Particle Purification
 
 

The purification of viruses and virus-like particles presents several challenges due to their large size and complexity. These studies explain the importance of resin screening and the scale-up purification strategies of a retrovirus and Influenza A H1N1 subtype in a single purification step.

Metal Affinity Chromatography For Purification
 
 

A high-capacity IMAC resin is designed for robust, scalable purification of histidine-tagged proteins and proteins naturally rich in histidine or cysteine residues. A case study demonstrates that the resin provides efficient capture of the tagged protein at high flow rates while maintaining high binding capacity. In another case study, an enzyme is immobilized to the IMAC resin to isolate the product by filtration.

Purification Of A Low-Expressing Recombinant Protein
 
 

In this case study, multiple chromatography resins were screened for the capture step of this recombinant protein with goals to minimize fermentation dilution and increase purity and recovery. Evaluation of modifiers in the elution buffer was found to enhance recovery and purity when using a hydrophobic AEX chromatography resin.

Purification Of IgM
 
 

Purification of large biomolecules such as IgM can be limiting with traditional ion exchange resins due to resin pore size. In this study, a high-capacity ion exchange resin was used that provided high dynamic binding capacity at fast flow rates delivering excellent process economics. The optimized pore size allows easy access and adsorption of large biomolecules.

Sample Resins For Metal Removal And Buffer Preparation
 
 

Removing contaminants such as metals or detergents is a commonly used step in buffer preparation for bioprocessing. Chromatography resins provide operational flexibility to remove these contaminants effectively in batch or packed column formats. Bio-Rad resins are highly purified and sized to provide consistent, reproducible results across buffer preparation from lab- to production-scale. They are available in a variety of particle (mesh) sizes and chemistries. Request samples today.

Resin Screening Tools
 

Screening chromatography resins can accelerate method development and optimize downstream purification workflows for biomolecules. Request Samples.

 

Industry Event

December 1, 2021 at 1:00 p.m. CET | 7:00 a.m. EDT
mAb Purification Webcast: A Mixed-Mode Approach — Alternative to a Three-Step Purification Process
Register Now.