Dilution Of Multiple Buffer Concentrates With One Single-Use Flow Path

Buffers are used in a variety of bioprocessing steps, ranging across multiple product recovery, purification and formulation processes such as diafiltration and chromatography. The use of buffers within downstream processing is particularly important as they are used to protect proteins from variations in pH and to ensure that proteins interact correctly with various media to maximize performance of the specified purification process.

To optimize the buffer process, the Allegro Connect buffer management system (BMS) can be used to provide users with an efficient and optimized design for buffer management. The Allegro Connect BMS is an automated in-line buffer dilution system designed to manage the production of diluted process buffers ondemand and just-in-time from 5x to 20x stock buffer concentrates to feed various unit operations. The system consequently reduces the operator labor time, the facility space requirements and logistical bottlenecks when compared to traditional buffer management approaches, improving overall flexibility and ease-of-use.

Explore findings from a study evaluating the capacity for the Allegro Connect BMS to produce multiple diluted buffer solutions from stock buffer concentrates using one single-use flow path for bioprocessing. Results from the study demonstrate the system functionality to switch between multiple different buffer concentrates with minimal downtime on the system using the same single-use flow path.