Application Note

Cultivation Of Sf-9 Insect Cells And SEAP Expression In The Finesse «SmartGlass» Bioreactor

Source: Thermo Fisher Scientific

This application note describes the cultivation of Sf-9 suspension cells in the Finesse «SmartGlass» vessel bioreactor having a maximum working volume of 2.0 L. Using serum-free medium without components of animal origin viable cell densities of up to 4.964∙106 cells∙mL-1 were achieved. The SEAP expression was induced by the baculovirus expression vector system, which provided maximum SEAP activities of 56.4 U∙mL-1. By Stephan C. Kaiser, Ina Dittler, Nina Steiger and Dieter Eibl