Application Note

Comparing Recombinant Protein Production With A Shake Flask And A Bioreactor

Source: GE Healthcare Life Sciences

Comparing Recombinant Protein Production With A Shake Flask And A Bioreactor

This application note compares cell growth and recombinant protein production of multiple Chinese hamster ovary (CHO) cell clones when cultured in HyClone ActiPro basal medium and Cell Boost 7a and 7b feeding supplements. Results from shake flask cultures show high viable cell densities and protein production. In addition, scalability of the culture process was demonstrated in 2 and 50 L bioreactor cultures. Cell density and protein production were shown to be comparable between scales. Protein yields reached as high as 5 g/L in shake flasks, and as high as 5.5 g/L in bioreactor cultures.