The current evolution to high cell density and high-product titer monoclonal antibody cell cultures is placing a larger burden on traditional downstream clarification and purification operations. To alleviate this bottleneck, various types of pre-treatment are being employed to make the clarification process more efficient.
Polycationic polymer modified poly (allyl amine) [mPAA] and poly (diallyldimethyl-ammonium chloride) [pDADMAC] are very effective flocculation polymers that can be used as a pre-treatment solution when used in combination with our Clarisolve® depth filters. The Clarisolve® depth filter is a primary clarification tool with a gradient density structure specifically designed to the particle size distributions of pre-treated feed streams. With improved volumetric capacity and reduced turbidity over currently available depth filters, the clarification step of pre-treated feeds can be processed in a significantly reduced footprint.
Together, the Clarisolve® mPAA and pDADMAC polymers, and Clarisolve® depth filters provide an effective solution for harvesting high cell density cultures that can be easily incorporated into current clarification platforms.