Cell Culture Media Prep: Filter Selection And Cell Culture Performance

Membrane filtration of cell culture media is a robust, easy to implement, time-tested solution that significantly reduces the risk of bioreactor contamination. Selecting a membrane filter is highly dependent on risk analysis, and the filter capacity for your specific media.

Although smaller pore size filters reduce the risk of bioreactor contamination, increased safety assurance may result in lower filtration flux and filter capacity. Membrane composition, structure, surface chemistry and layering results in permeability and capacity differences between filters. These differences affect filtration area requirements, footprint and cost of goods. While important, equally important is the cell culture performance in filtered media; it is critical that any filtration solution meets performance objectives in terms of viable cell density, and productivity.

This poster shows how membrane filter pore size and materials affect CHO cell culture media filtration and performance.

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