As the global demand for biopharmaceuticals continues to rise, bioprocessing is evolving with a focus on maximizing productivity upstream through improvements in cell line development and cell culture media optimization. However, efficiently processing cell culture media can be challenging as many of these new media are highly concentrated and complex, with components that may lead to premature fouling of membrane filters.
Membrane filtration is a robust, easy-to-implement, time-tested solution for processing cell culture media that significantly reduces the risk of bioreactor contamination. Selecting a membrane filter for processing cell culture media is influenced by the desired level of microbial retention, compatibility of the filter materials with the fluid stream, and the filter capacity for your specific media.
Upstream bioprocessing is evolving with improvements in cell line development and cell culture media optimization. This application note provides an overview of different filtration options in terms of microbial retention and filtration capacity and provides filter sizing recommendations for MilliporeSigma catalog media and additives. Increased understanding of these different criteria should help biomanufacturers select the right filter for their process needs.