Application Note

Automated Multidimensional Chromatography Purification At The Push Of A Button

Source: Bio-Rad Laboratories, Inc.

By Jeff Habel, Philip Chapman, and Gunjan Choudhary, Bio-Rad Laboratories

Protein purification often requires iterations of individual column purifications, sample fractionation and visualization, and fraction pooling for the next column.  Significant time is spent developing the purification strategy and using that information for subsequent protein purification cycles.  A well-designed Multi-D method utilizes optimized individual column methods to create an automated and highly reproducible multistep procedure.