By Corning Incorporated
The Corning High Yielding Performance Flask (HYPERFlask®) cell culture vessel was developed to increase the number and quality of cells required by scientists in various types of laboratories, including High Throughput Screening. The multilayered HYPERFlask vessel uses a gas permeable film to provide gas exchange between the internal culture environment and the external atmospheric environment. The unique film design gives a 1720 cm2 surface area, which is approximately 10 times that of a normal T175 flask. The HYPERFlask vessel can be completely filled with medium and capped with a plug sealed cap that does not require venting.
Scientists interested in automating the HYPERFlask vessel process have limited options in terms of technology. This protocol will describe one automation method using the Pipeline Dispenser from Essen Instruments, Inc. (Ann Arbor, MI). The Pipeline Dispenser provides a fast, accurate and efficient method to transfer medium into the HYPERFlask vessel, saving time and reducing fatigue.