Monitor Nutrients And Metabolites In Cell Culture Media To Improve Yield

There is a need for advanced process analytic technologies that can monitor cell media nutrients and cellular metabolites alongside bioreactors in process development labs. Biochemical analyzers based on enzymatic assays deliver useful data, but they do not give a complete picture of the bioreactor state as they are limited to a small number of analytes. Comprehensive spent media analysis of amino acids and other media components is bottlenecked by core lab turn-around times which slow media optimization and process decisions severely. To elevate process development decisions to real-time, researchers require new analytical platforms that simplify analysis and accelerate delivery of actionable intelligence.

Traditional spent media analysis requires at least two separate HPLC runs for the coverage of both amino acids, vitamins, and other components. The Rebel™ eliminates this complexity by analyzing each sample for amino acids, vitamins, amines and dipeptides in a single run. In this experiment, See how an at-line analyzer removes process bottlenecks and speeds up process development.