Article: A Review of Secondary Retention Phenomena in the Ion Exchange Chromatography of Proteins
Source: Avantor Performance Materials, Inc.
This review focuses on the major physiochemical forces responsible for secondary retention phenomena in the ion exchange chromatography of proteins. The underlying physiochemical and mechanistic aspects of hydrogen bonding and vand er Waals forces and hydrophobic and hydrophilic interactions will be discussed, particularly with the respect to their influence on protein retention behavior.
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