Application Note

Analyzing Protein Interactions Without Radiochemicals or Fluorescent Labels

Source: Bio-Rad Laboratories, Inc.

Virtually every cellular structure and process depends on protein interactions. DNA replication and transcription, RNA splicing and translation, protein modification and secretion, cell cycle control and apoptosis, cell growth and intermediary metabolism, signal transduction, and gene expression — all exploit intricate protein interactions for the execution and maintenance of complex cellular life processes, making the analysis of protein interactions an especially valuable area of research.

Surface plasmon resonance (SPR) optical biosensing is an analytical technique that requires neither radiochemical nor fluorescent labels to provide real-time data on the affinity, specificity, and interaction kinetics of protein interactions. SPR biosensors generate the interaction data required to establish the functional roles of proteins, understand cellular function, and develop new drugs to treat disease (Rich and Myszka 2005).