Technical Note: Achieving Ultra-High-Speed Analysis With LC/MS/MS

Since analysis times can be accelerated at least 10-fold compared with conventional systems, systems that provide ultra-high-speed analysis can improve analysis operations. Although it may seem that conventional LC/MS/MS systems could achieve ultra-high-speed analysis simply by switching to a sub-2 µm column, is it really so?

This report addresses the problems associated with conventional LC/MS/MS systems in achieving ultra-high-speed analysis, and introduces a resolution to these problems.

The first consideration is the LC instrument. Shortened analysis times can be achieved by (1) reducing the column volume (e.g., shortening the length), or (2) increasing the mobile phase flow rate. However, separation will deteriorate with a typical column. A better approach is to reduce the column particle size and increase the number of theoretical plates. Sub-2 µm particle sizes are now being used worldwide. And since there is no change in theoretical plate height when the flow rate is increased, separation is maintained.