A Model System For Fast Screening Of Optimal Protein Purification Conditions

We developed a model system for screening optimal protein purification conditions on a mixed-mode cation exchange resin using a statistical software–generated design of experiment (DOE) model with ChromLab Software’s Multivariable Scouting (MVS) function on the NGC Chromatography System. We evaluated combinations of buffers with varying pH and conductivity to identify optimal binding and elution conditions. An automated tandem and multivariable scouting method was created to identify ideal IgG binding and elution conditions that included different Bio-Rad columns for capture, buffer exchange, and screening interaction modes. With a fully automated screening process, identification of optimal conditions was reduced from the standard five days to less than 18 hours.