This application note describes mixing and heating-cooling characterization data for the Xcellerex XDM/ XDUO 500 single-use mixer. A design of experiments (DoE) approach to liquid-liquid mixing was successfully applied to establish a statistical model to predict the mixing time throughout the working range. Liquid-liquid mixing times as low as 17 s were observed at the nominal volume (500 L) and highest viscosity tested (20 cP). Further, mixing of two different solids, PBS and NaCl, was tested. Mixing to 95% homogeneity (tm95) of both solids was reached within 4 min at 150 rpm. Heating of liquid from 5°C to 20°C and 20°C to 37°C was achieved within 2 h for all tested volumes (110, 305, and 500 L). Cooling from 37°C to 20°C and 20°C to 5°C was achieved within 4 h.