Parallel Cultivation Of Microorganisms: Using Rigid-Wall Single-Use Bioreactors
In recent years, single-use bioreactor solutions have been successfully established for animal and human cell culture processes. Such technology is under investigation for microbial applications. Sebastian Kleebank is development and application laboratory manager for the Eppendorf line of bioprocess products. His cell culture and microbial laboratory performs parallel cultivations for prototype testing, user training, and software development.
WHITE PAPERS & CASE STUDIES
High Density Hybridoma Cell Culture In A Packed-Bed Bioreactor
Packed-bed bioreactor cell culture is generally accepted as one of the best methods to simulate the conditions of animal cell growth in vivo since cells are maintained in a low-shear environment with constant refreshment of nutrients and removal of waste. By Ray Rose, Stacey Willard, and Ma Sha
Anaerobic Yeast Fermentation Using Redox Control
Metabolic activity of microorganisms depends on many factors, including oxidation and reduction reactions, or the “redox potential” of the culture environment.
Rip It Up And Start Again: Developing A Bioreactor For Vaccines
While incremental advances can create solutions to technological problems, sometimes the only way to achieve the required result is to rip up the old rulebook and start from scratch. That’s the approach ATMI LifeSciences took in developing a bioreactor for vaccine manufacture. By Jose Castillo, Director of Cell Culture Technologies at ATMI LifeSciences
Cultivation Of Human CAP Cells: Evaluation Of Scale-Down Capabilities
CEVEC has established a master cell bank (MCB) of CAP cells growing in suspension, tested and certified according to ICH guidelines and European Pharmacopeia. Simple and reliable protocols allow for the fast generation of customized producer cell lines for pharmaceutically relevant proteins based on the parental permanent CAP cells under controlled and optimized conditions.
Process For Scale-Up Of Adherent Vero Cells From Static Cell Factories To Influenza Production
In cell culture-based vaccine production, scale-up of adherent cells is challenging. This study shows a process for scaling up adherent Vero cells from static cell factories to influenza production at 50 L scale using WAVE Bioreactor™ systems and ReadyToProcess single-use equipment.
A Comparison Of Performance Using Three Bioreactor Systems
Eppendorf has introduced a new benchtop cell culture bioreactor that combines the benefits of single-use technology with the trusted performance and true scalability of a stirred-tank design. CelliGen™ BLU eliminates the need for autoclaving and cleaning, reduces validation requirements, provides rapid turn-around between runs, minimizes startup costs, and significantly reduces the risk of contamination because the culture vessel is only used once and then discarded.