If you’ve ever walked out into an ocean, you’ve felt the impact of waves propagating on the surface of the water. Hit from the back and you fall forward; hit from the front and you’ll find yourself face first in the water. However, in very choppy waters, you could potentially get hit from the front by waves coming from the sea, while simultaneously being hit from the back by waves bouncing off of the shore line. If these waves are timed correctly, the equal and opposite forces could trap you in a stable position; this is referred to as a standing wave. FloDesign Sonic uses a specially-designed, 3D version of a standing wave to act as an invisible barrier (or filter) to catch cells while the remaining liquid and materials pass through. The cells group inside the standing wave and precipitate out of the fluid flow, where FloDesign Sonic uses specially-designed fluidics to continuously draw off the concentrated cells.
Fundamentals Of Viral Clearance Studies Part 4: Considerations For Evaluating Viral Clearance By Chromatography Steps
Chromatography steps are typically the backbone of the purification process for a biopharmaceutical product, and are usually included in the evaluation of the viral reduction capacity of a manufacturing process. In viral clearance studies of chromatography steps, the capacity of the chromatography resin to provide physical separation of the spiked virus from the product fraction is determined. Viral reduction by chromatography steps is considered less robust than by other less complex steps, such as inactivation steps. By Kathryn Martin Remington, Ph.D., Principal Scientist for Clearance Services, BioReliance
Do Chinese Hamster Ovarian (CHO) Cells Specimens Require An Import/Export License?
On December 21, 2012, the U.S. Fish and Wildlife Service (FWS) addressed an issue that has been of significant debate among importers of Chinese Hamster Ovary (CHO) cell specimens and trade experts. FWS informed the trade community that only shipments of actual CHO “cell”, “cell cultures” and “cell lines” are regulated by the agency and require an import/export license for trade. By Reynaldo Roman for Marken
Fundamentals Of Viral Clearance Studies Part 1: Selection Of A Viral Panel And An Appropriate Virus Spike
A viral clearance study is designed to experimentally demonstrate the virus reduction that could be achieved by selected steps in a biopharmaceutical manufacturing process in the unlikely event that process intermediates were to be contaminated by an infectious virus. By Kathryn Martin Remington, Ph.D., Principal Scientist for Clearance Services, BioReliance
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Time Savings With Simplified Cell Culturing
This application note describes how ReadyToProcess WAVE 25 can help reduce hands-on time as much as 25% in fed-batch culturing, while delivering excellent culture performance. For an optimized perfusion application, the use of intelligent control software enabled accurate regulation of process parameters. Over 100 × 106 viable cells/mL was easily achieved in seven days.
Cultivation Of Aggressive Microbial Cultures Of Pichia And Bacillus
Advances in single-use bioprocess technology have led to the rapid adoption of single-use bioreactors (S.U.B.s) across a broad range of applications within the biotechnology industry.
Scale-Up Of Microbial Fermentation Using Recombinant E. Coli
Fermentation production facilities can utilize the Thermo Scientific™ HyPerforma™ Single-Use Fermentor (S.U.F.) instead of traditional stainless steel cleaning-in-place/ sterilization-in-place (CIP/SIP) fermentor vessels without modifying their existing procedures.
Use Of Phase Contrast Imaging To Track Morphological Cellular Changes Due To Apoptotic Activity
Phenotypic screening, or the determination of the effects (phenotypes) that a molecule has on a cell, tissue, or whole organism, dates back to earliest drug discovery efforts. By Brad Larson and Peter Banks, Applications Department, BioTek Instruments, Inc.
Z-Stacking Of Single Plane Digital Widefield Fluorescent Images
Incorporation of Cytation™ Cell Imaging Multi-Mode Readers and Gen5™ Data Analysis Software to Create Deconvoluted, Stacked Images of 3D Cellular Structures. By Brad Larson and Peter Banks, Applications Department, BioTek Instruments, Inc.
Adaption Of The BioLector Technology To Anaerobic Requirements
Biorefineries have reignited interest in anaerobic fermentations with biobutanol production being the principle driver. Already during the First World War Biobutanol and acetone were produced in Clostridium acetobutylicum.
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CELL CULTURE MEDIAMore Cell Culture Media
Biologics Research Promises To Bolster Future Of Medicine
America's biopharmaceutical companies are using biological processes to develop 907 medicines and vaccines targeting more than 100 diseases, according to a new report released today by the Pharmaceutical Research and Manufacturers of America (PhRMA).
Baxter Initiates Phase I Clinical Trial With Anti-MIF Antibody In Patients With Solid Tumors
Baxter International Inc. has begun dosing patients with malignant solid tumors in a Phase I clinical trial of a monoclonal antibody, representing the company’s efforts to extend its oncology portfolio with advanced biological research and development.
Eureka Genomics Awarded Multi Year Contract To Assist Military Laboratory With DNA Sequencing
Based on collaborative work done for the past twelve months with the Armed Forces DNA Identification Laboratory (AFDIL), Eureka Genomics has been awarded a multi-year contract with AFDIL to provide forensic service support that should advance efforts to identify unknown remains from the Korean War.
Lilly's Anti-IL-17 Monoclonal Antibody, Ixekizumab, Met Primary Endpoint In Phase II Study In Patients With Chronic Plaque Psoriasis New Phase II data, published today in the New England Journal of Medicine, showed that Eli Lilly and Company's (NYSE: LLY) ixekizumab (pronounced ix" e kiz' ue mab, previously known as LY2439821), an anti-IL-17 monoclonal antibody, met its primary endpoint in patients with moderate-to-severe plaque psoriasis, with significantly more patients achieving at least a 75 percent improvement in Psoriasis Area and Severity Index (PASI) scores from baseline (PASI 75) compared with placebo at week 12.
Argen-X Launches NHance™ Technology To Generate Better Human Antibody Therapies arGEN-X announces today the launch of NHance™, its latest cutting-edge technology enabling the creation of truly differentiated human antibody therapeutics.
Roche´s MagNA Pure LC 2.0 System For Monitoring DNA In Therapeutic Proteins And Monoclonal Antibody Drugs To ensure product safety, clearance of host-cell DNA is essential when manufacturing therapeutic proteins and monoclonal antibody (mAB) drugs.
Xencor Receives U.S. Patent For Monoclonal Antibody Engineering That Extends Duration Of Action Xencor, Inc., a company using its proprietary Protein Design Automation® (PDA) platform technology to engineer next-generation antibodies, announced today that the U.S. Patent and Trademark Office has issued Xencor a broad patent covering optimized monoclonal antibody variants and their application for extending in vivo half-life of antibodies.
DSM Expands Its Innovative Biomanufacturing Technology Portfolio With The Introduction Of The New Kremer Method™ Royal DSM, the global Life Sciences and Materials Sciences company, today announced the introduction of the Kremer Method™, an innovative proprietary technology developed by DSM scientists which improves downstream processing of monoclonal antibodies (mAbs) for faster and more cost effective production of biopharmaceuticals.
Cell culture is a complex, highly structured process for growing cells, under strictly controlled conditions, outside of their normal environment. Cell cultures stilluse cultures of cells on flat plastic dishes.
This is referred to as two-dimensional (2D) cell culture. Aside from using Petri dishes for growing cells, scientists have for a long time, grown cells within biologically-derived matrices such as collagen or fibrin.
Today, more and more 3d cell cultures are being used because they more closely resemble the in vitro cell growth environment. Most 3d cell cultures in use today are designed for stem cell research, tissue engineering and drug discovery. As the field continues to grow and expand, 3d cell culture availability will likely expand to include other cell culture related fields.
For non-adhesive cells suspension cell cultures are used. In these cultures a cell is placed in the liquid suspension, stirred with a magnetic stirrer to agitate the cell and make it float freely in the suspension. The cell grows, divides and spreads throughout the suspension.
Cell culture refers to the culturing of cells derived from multi-cellular eukaryotes (cells with a nucleus), primarily animal cells. However cell cultures also exist for plants, fungi and microbes that include viruses, bacteria and microorganisms. Cell culture shares closely related methodology with tissue culture and organ culture.
You can separate cells from tissues for use in cultures several ways. Cells can be purified from blood but only white cells will grow in a culture. Mononuclear cells can be released from soft tissue using enzymes that break the cells away from their substrate or matrix. Pieces of tissue can also be placed in a growth media and the cells that grow from it can be used for cell cultures.