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FEATURED ARTICLES

Parallel Cultivation Of Microorganisms: Using Rigid-Wall Single-Use Bioreactors

In recent years, single-use bioreactor solutions have been successfully established for animal and human cell culture processes. Such technology is under investigation for microbial applications. Sebastian Kleebank is development and application laboratory manager for the Eppendorf line of bioprocess products. His cell culture and microbial laboratory performs parallel cultivations for prototype testing, user training, and software development.

  • Tapping Into Metabolites To Overcome R&D Challenges And Develop Novel Biomarkers: Part 1
    Tapping Into Metabolites To Overcome R&D Challenges And Develop Novel Biomarkers: Part 1

    As we continue to strive to mine the genome for clues that can assist in understanding susceptibility to disease, selection of better targets for combating disease, and the biomarkers delineating response, a key source of data that can either lead or strengthen this pursuit is not always part of this equation. So what is this data and why would one ever exclude it?

  • At Ferring Pharmaceuticals, Peptides And Proteins Make Significant Advances
    At Ferring Pharmaceuticals, Peptides And Proteins Make Significant Advances

    Dr. Keith James joined Ferring Pharmaceuticals as VP of Therapeutic Innovation in early 2012. In December 2012 he was promoted to president of the Ferring Research Institute and SVP of R&D for Ferring Pharmaceuticals. Prior to joining Ferring, Dr. James spent 29 years working for Pfizer, with significant time spent in discovery research. At the 2013 Oligonucleotide & Peptide Based Therapeutics Congress he will speak on recent progress and future prospects in the peptide and protein therapeutic space. I recently spoke with Dr. James to get an overview of the potential in this market.

  • Fundamentals Of Viral Clearance Studies Part 4: Considerations For Evaluating Viral Clearance By Chromatography Steps
    Fundamentals Of Viral Clearance Studies Part 4: Considerations For Evaluating Viral Clearance By Chromatography Steps

    Chromatography steps are typically the backbone of the purification process for a biopharmaceutical product, and are usually included in the evaluation of the viral reduction capacity of a manufacturing process.   In viral clearance studies of chromatography steps, the capacity of the chromatography resin to provide physical separation of the spiked virus from the product fraction is determined.  Viral reduction by chromatography steps is considered less robust than by other less complex steps, such as inactivation steps.  By Kathryn Martin Remington, Ph.D., Principal Scientist for Clearance Services, BioReliance

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WHITE PAPERS & CASE STUDIES

  • Adaption Of The BioLector Technology To Anaerobic Requirements
    Adaption Of The BioLector Technology To Anaerobic Requirements

    Biorefineries have reignited interest in anaerobic fermentations with biobutanol production being the principle driver. Already during the First World War Biobutanol and acetone were produced in Clostridium acetobutylicum.

  • Batch Culture Of CHO Cells In The Multifors Cell
    Batch Culture Of CHO Cells In The Multifors Cell

    The Multifors Cell can be used to easily optimise development processes by parallel cultivation of animal cell cultures. Cultivation of the CHO (Chinese hamster ovary) cell line in the Multifors Cell bioreactor (INFORS HT, CH-Bottmingen) is described in the following as an example of batch cultivation of parallel samples.

  • Optimization Of Monoclonal Antibody Production Employing Noninvasive Sensor Technology
    Optimization Of Monoclonal Antibody Production Employing Noninvasive Sensor Technology

    Large scale high density fermentation and high yield production of monoclonal antibodies are major aims of process development in biopharmaceutical industry.

  • High Density Hybridoma Cell Culture In A Packed-Bed Bioreactor
    High Density Hybridoma Cell Culture In A Packed-Bed Bioreactor

    Packed-bed bioreactor cell culture is generally accepted as one of the best methods to simulate the conditions of animal cell growth in vivo since cells are maintained in a low-shear environment with constant refreshment of nutrients and removal of waste. By Ray Rose, Stacey Willard, and Ma Sha

  • Anaerobic Yeast Fermentation Using Redox Control
    Anaerobic Yeast Fermentation Using Redox Control

    Metabolic activity of microorganisms depends on many factors, including oxidation and reduction reactions, or the “redox potential” of the culture environment.

  • Skip Steps In Cell Culturing

    With Cell Culture Flask Adapters, the culture can be centrifuged directly in the flask. Data illustrates that cell yield, cell viability, and endpoint analysis results are comparable when cell cultures are processed traditionally or centrifuged directly in the flask using Cell Culture Flask Adapters.

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PRODUCTS & SERVICES

Hyclone Cell Culture Media, Sera And Process Liquids

Hyclone Cell Culture Media, Sera And Process Liquids

In March 2014, GE Healthcare Life Sciences announced the completion of the acquisition of the HyClone cell culture media business  from Thermo Fisher Scientific.  The addition of the HyClone portfolio significantly broadens our existing capabilities in life sciences research and bioprocessing. The result is a comprehensive portfolio of products and services across the whole biomanufacturing workflow, from upstream to downstream, from discovery to commercialization.

Read-To-Use Medium: BalanCD™ MDCK

Read-To-Use Medium: BalanCD™ MDCK

BalanCD™ MDCK has been designed for producing viruses in Madin-Darby Canine Kidney cells (MDCK) cultures. This medium is chemically-defined and animal-component-free.

Flexsafe Single-Use Bags

Flexsafe Single-Use Bags

Standard Flexsafe 2D validation bags made of the new polyethylene S80 film are designed for small scale investigations and for the validation of the Flexsafe bag family. They are available in 150 mL and 500 mL volumes to support end users’ process development and validation testing. Flexsafe bags have been designed by combining material science and a Quality by Design approach to provide outstanding cell growth, robustness and assurance of supply for all process steps of biomanufacture.

BioLector® Pro

BioLector® Pro

The Biolector® Pro is the first microbioreactor system combining the proven, scalable BioLector® technology with an innovative microfluidic chip.

Animal Product Free Microcarriers

Animal Product Free Microcarriers

SoloHill Animal Product Free Microcarriers offer high performance options for researchers wishing to limit or remove animal sources from their processes. They address the worldwide concern with the spread of bovine spongiform encephalopathy (BSE) from cattle to humans as vCJD (variant Creutzfeldt-Jakob disease) which has driven the world's regulatory agencies to strongly suggest the eventual removal of all animal proteins from pharmaceutical manufacturing processes.

Minitron Incubator Shaker

Minitron Incubator Shaker

With the launch of the Minitron incubator shaker, INFORS HT is following up on the worldwide success of the Multitron incubator shaker. The Minitron has the same outstanding characteristics as its big brother, the Multitron, but on a smaller scale.

2D Bar Code Reader For Storage Vials

2D Bar Code Reader For Storage Vials

WHEATON understands that when it comes to BioBanking, sample integrity means everything.

Sample Batching And Storage Boxes

Sample Batching And Storage Boxes

WHEATON BioBanking Solutions include color coded storage and freezer boxes to make your sample storage and tracking solutions customized and easily identifiable.

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CELL CULTURE MEDIA

Advances In Protein Production Bring New Options For Flexibility In Bioprocessing

Advances In Protein Production Bring New Options For Flexibility In Bioprocessing

Advances in protein production, including biomaterials and cell culture, have resulted in new methods for bioprocessing that can increase both yield and quality as well as decrease cost and time. Trisha Gladd, editor of Pharmaceutical Online and Bioprocess Online, spoke with Dr. Mike Ultee, Chief Scientific Officer at Gallus Biopharmaceuticals, about not just these new options but also the future of bioprocessing.

 

Measure More Samples Faster: NanoDrop 2000C Video

NanoDrop instruments revolutionized the way scientists measure their precious samples. The patented* sample retention technology eliminates extra steps and allows scientists to quickly measure more samples. That's why the NanoDrop 2000c is the gold standard for microvolume DNA, RNA, and protein quantification.

Easily Switch Between Washing And Filtration With An Interchangeable Microplate Washer

The Hydroflex microplate washer is a modular and upgradeable platform is ideal for a wide range of cell-, enzyme- and DNA-based applications in academia, biotech, pharma and clinical diagnostics, reflecting over 30 years of Tecan expertise in advanced liquid handling.

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Cell Culture
Cell culture is a complex, highly structured process for growing cells, under strictly controlled conditions, outside of their normal environment. Cell cultures stilluse cultures of cells on flat plastic dishes.
This is referred to as two-dimensional (2D) cell culture. Aside from using Petri dishes for growing cells, scientists have for a long time, grown cells within biologically-derived matrices such as collagen or fibrin.
Today, more and more 3d cell cultures are being used because they more closely resemble the in vitro cell growth environment. Most 3d cell cultures in use today are designed for stem cell research, tissue engineering and drug discovery. As the field continues to grow and expand, 3d cell culture availability will likely expand to include other cell culture related fields.
For non-adhesive cells suspension cell cultures are used. In these cultures a cell is placed in the liquid suspension, stirred with a magnetic stirrer to agitate the cell and make it float freely in the suspension. The cell grows, divides and spreads throughout the suspension.
Cell culture refers to the culturing of cells derived from multi-cellular eukaryotes (cells with a nucleus), primarily animal cells. However cell cultures also exist for plants, fungi and microbes that include viruses, bacteria and microorganisms. Cell culture shares closely related methodology with tissue culture and organ culture.
You can separate cells from tissues for use in cultures several ways. Cells can be purified from blood but only white cells will grow in a culture.  Mononuclear cells can be released from soft tissue using enzymes that break the cells away from their substrate or matrix. Pieces of tissue can also be placed in a growth media and the cells that grow from it can be used for cell cultures.