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FEATURED ARTICLES

Riding An Acoustic Wave Into The Future Of Single Use

If you’ve ever walked out into an ocean, you’ve felt the impact of waves propagating on the surface of the water. Hit from the back and you fall forward; hit from the front and you’ll find yourself face first in the water. However, in very choppy waters, you could potentially get hit from the front by waves coming from the sea, while simultaneously being hit from the back by waves bouncing off of the shore line. If these waves are timed correctly, the equal and opposite forces could trap you in a stable position; this is referred to as a standing wave. FloDesign Sonic uses a specially-designed, 3D version of a standing wave to act as an invisible barrier (or filter) to catch cells while the remaining liquid and materials pass through. The cells group inside the standing wave and precipitate out of the fluid flow, where FloDesign Sonic uses specially-designed fluidics to continuously draw off the concentrated cells.

  • Fundamentals Of Viral Clearance Studies Part 4: Considerations For Evaluating Viral Clearance By Chromatography Steps
    Fundamentals Of Viral Clearance Studies Part 4: Considerations For Evaluating Viral Clearance By Chromatography Steps

    Chromatography steps are typically the backbone of the purification process for a biopharmaceutical product, and are usually included in the evaluation of the viral reduction capacity of a manufacturing process.   In viral clearance studies of chromatography steps, the capacity of the chromatography resin to provide physical separation of the spiked virus from the product fraction is determined.  Viral reduction by chromatography steps is considered less robust than by other less complex steps, such as inactivation steps.  By Kathryn Martin Remington, Ph.D., Principal Scientist for Clearance Services, BioReliance

  • Do Chinese Hamster Ovarian (CHO) Cells Specimens Require An Import/Export License?

    On December 21, 2012, the U.S. Fish and Wildlife Service (FWS) addressed an issue that has been of significant debate among importers of Chinese Hamster Ovary (CHO) cell specimens and trade experts. FWS informed the trade community that only shipments of actual CHO “cell”, “cell cultures” and “cell lines” are regulated by the agency and require an import/export license for trade. By Reynaldo Roman for Marken

  • Fundamentals Of Viral Clearance Studies Part 1: Selection Of A Viral Panel And An Appropriate Virus Spike
    Fundamentals Of Viral Clearance Studies Part 1: Selection Of A Viral Panel And An Appropriate Virus Spike

    A viral clearance study is designed to experimentally demonstrate the virus reduction that could be achieved by selected steps in a biopharmaceutical manufacturing process in the unlikely event that process intermediates were to be contaminated by an infectious virus. By Kathryn Martin Remington, Ph.D., Principal Scientist for Clearance Services, BioReliance

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WHITE PAPERS & CASE STUDIES

  • Time Savings With Simplified Cell Culturing
    Time Savings With Simplified Cell Culturing

    This application note describes how ReadyToProcess WAVE 25 can help reduce hands-on time as much as 25% in fed-batch culturing, while delivering excellent culture performance. For an optimized perfusion application, the use of intelligent control software enabled accurate regulation of process parameters. Over 100 × 106 viable cells/mL was easily achieved in seven days.

  • Cultivation Of Aggressive Microbial Cultures Of Pichia And Bacillus
    Cultivation Of Aggressive Microbial Cultures Of Pichia And Bacillus

    Advances in single-use bioprocess technology have led to the rapid adoption of single-use bioreactors (S.U.B.s) across a broad range of applications within the biotechnology industry.

  • Scale-Up Of Microbial Fermentation Using Recombinant E. Coli
    Scale-Up Of Microbial Fermentation Using Recombinant E. Coli

    Fermentation production facilities can utilize the Thermo Scientific™ HyPerforma™ Single-Use Fermentor (S.U.F.) instead of traditional stainless steel cleaning-in-place/ sterilization-in-place (CIP/SIP) fermentor vessels without modifying their existing procedures.

  • Use Of Phase Contrast Imaging To Track Morphological Cellular Changes Due To Apoptotic Activity
    Use Of Phase Contrast Imaging To Track Morphological Cellular Changes Due To Apoptotic Activity

    Phenotypic screening, or the determination of the effects (phenotypes) that a molecule has on a cell, tissue, or whole organism, dates back to earliest drug discovery efforts. By Brad Larson and Peter Banks, Applications Department, BioTek Instruments, Inc.

  • Z-Stacking Of Single Plane Digital Widefield Fluorescent Images
    Z-Stacking Of Single Plane Digital Widefield Fluorescent Images

    Incorporation of Cytation™ Cell Imaging Multi-Mode Readers and Gen5™ Data Analysis Software to Create Deconvoluted, Stacked Images of 3D Cellular Structures. By Brad Larson and Peter Banks, Applications Department, BioTek Instruments, Inc.

  • Adaption Of The BioLector Technology To Anaerobic Requirements
    Adaption Of The BioLector Technology To Anaerobic Requirements

    Biorefineries have reignited interest in anaerobic fermentations with biobutanol production being the principle driver. Already during the First World War Biobutanol and acetone were produced in Clostridium acetobutylicum.

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PRODUCTS & SERVICES

Mix-Mode Chromatography Media

Mix-Mode Chromatography Media

Mixed-mode chromatography media is a top tier selection for the manipulation, purification and handling of molecules; providing an unmatched level of separation properties and resolution. The combination of cation exchange media and metal affinity media properties, allows multimodal chromatography to provide an access route for biomolecules to separate and the use of alternative chromatographic methods make these biomolecules seem homogenous.

HyPerforma™ Single-Use Fermentor (S.U.F.)

HyPerforma™ Single-Use Fermentor (S.U.F.)

The S.U.F. is engineered for optimal oxygen mass transfer, mixing, and temperature control, and performs comparably to traditional stainless steel systems. Using traditional, fundamental engineering principles, scale-up and process development work is streamlined – offering you cost savings and efficiency. 
 

Hyclone Cell Culture Media, Sera And Process Liquids

Hyclone Cell Culture Media, Sera And Process Liquids

In March 2014, GE Healthcare Life Sciences announced the completion of the acquisition of the HyClone cell culture media business  from Thermo Fisher Scientific.  The addition of the HyClone portfolio significantly broadens our existing capabilities in life sciences research and bioprocessing. The result is a comprehensive portfolio of products and services across the whole biomanufacturing workflow, from upstream to downstream, from discovery to commercialization.

BalanCD® MDCK: Chemically-Defined Medium for Vaccine Manufacturing

BalanCD® MDCK: Chemically-Defined Medium for Vaccine Manufacturing

BalanCD® MDCK has been designed for producing viruses in Madin-Darby Canine Kidney cells (MDCK) cultures. This medium is chemically-defined and animal-component-free.

Flexsafe Single-Use Bags

Flexsafe Single-Use Bags

Standard Flexsafe 2D validation bags made of the new polyethylene S80 film are designed for small scale investigations and for the validation of the Flexsafe bag family. They are available in 150 mL and 500 mL volumes to support end users’ process development and validation testing. Flexsafe bags have been designed by combining material science and a Quality by Design approach to provide outstanding cell growth, robustness and assurance of supply for all process steps of biomanufacture.

BioLector® Pro

BioLector® Pro

The Biolector® Pro is the first microbioreactor system combining the proven, scalable BioLector® technology with an innovative microfluidic chip.

Animal Product Free Microcarriers

Animal Product Free Microcarriers

SoloHill Animal Product Free Microcarriers offer high performance options for researchers wishing to limit or remove animal sources from their processes. They address the worldwide concern with the spread of bovine spongiform encephalopathy (BSE) from cattle to humans as vCJD (variant Creutzfeldt-Jakob disease) which has driven the world's regulatory agencies to strongly suggest the eventual removal of all animal proteins from pharmaceutical manufacturing processes.

Minitron Incubator Shaker

Minitron Incubator Shaker

With the launch of the Minitron incubator shaker, INFORS HT is following up on the worldwide success of the Multitron incubator shaker. The Minitron has the same outstanding characteristics as its big brother, the Multitron, but on a smaller scale.

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CELL CULTURE MEDIA

Advances In Protein Production Bring New Options For Flexibility In Bioprocessing

Advances In Protein Production Bring New Options For Flexibility In Bioprocessing

Advances in protein production, including biomaterials and cell culture, have resulted in new methods for bioprocessing that can increase both yield and quality as well as decrease cost and time. Trisha Gladd, editor of Pharmaceutical Online and Bioprocess Online, spoke with Dr. Mike Ultee, Chief Scientific Officer at Gallus Biopharmaceuticals, about not just these new options but also the future of bioprocessing.

 

Easily Switch Between Washing And Filtration With An Interchangeable Microplate Washer

The Hydroflex microplate washer is a modular and upgradeable platform is ideal for a wide range of cell-, enzyme- and DNA-based applications in academia, biotech, pharma and clinical diagnostics, reflecting over 30 years of Tecan expertise in advanced liquid handling.

Hydroflex™ Microplate Washer With Magnetic Bead Separation

The Hydorflex microplate washer has the option to incorporate a magnetic carrier and it can meet the demand of magnetic bead separation and an innovative, cutting edge method.

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Cell Culture
Cell culture is a complex, highly structured process for growing cells, under strictly controlled conditions, outside of their normal environment. Cell cultures stilluse cultures of cells on flat plastic dishes.
This is referred to as two-dimensional (2D) cell culture. Aside from using Petri dishes for growing cells, scientists have for a long time, grown cells within biologically-derived matrices such as collagen or fibrin.
Today, more and more 3d cell cultures are being used because they more closely resemble the in vitro cell growth environment. Most 3d cell cultures in use today are designed for stem cell research, tissue engineering and drug discovery. As the field continues to grow and expand, 3d cell culture availability will likely expand to include other cell culture related fields.
For non-adhesive cells suspension cell cultures are used. In these cultures a cell is placed in the liquid suspension, stirred with a magnetic stirrer to agitate the cell and make it float freely in the suspension. The cell grows, divides and spreads throughout the suspension.
Cell culture refers to the culturing of cells derived from multi-cellular eukaryotes (cells with a nucleus), primarily animal cells. However cell cultures also exist for plants, fungi and microbes that include viruses, bacteria and microorganisms. Cell culture shares closely related methodology with tissue culture and organ culture.
You can separate cells from tissues for use in cultures several ways. Cells can be purified from blood but only white cells will grow in a culture.  Mononuclear cells can be released from soft tissue using enzymes that break the cells away from their substrate or matrix. Pieces of tissue can also be placed in a growth media and the cells that grow from it can be used for cell cultures.