Downstream Processing White Papers and Case Studies
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Materials Of Construction For Biopharmaceutical Water Systems
1/15/2013
Stainless steels are uniquely qualified for bioprocessing applications not only because of their long service life, availability, and fabricability, but also because they are non-corroding, non-contaminant, can be polished to very smooth finishes, are strong and rigid, can withstand heat and chemical sterilization treatments, and are easily welded. By Michelle M. Gonzalez
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How To Characterize Multimodal Samples Using Particle Characterization Equipment
This application note will discuss the topic of size resolution in particle characterization and advantages and disadvantages of various techniques.
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CD69 Expression On Activated Lymphocytes Is Stable In Streck’s Cyto-Chex® BCT
7/16/2012
The purpose of this study was to determine if the activation marker, CD69, is preserved and stable in whole blood samples collected in Cyto-Chex® BCT. Previous studies have demonstrated CD marker stability of whole blood samples collected in Cyto-Chex BCT for up to 7 days when stored at room temperature (18-22°C). By Jodi Alt, Ph.D
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One Bioreactor System For The Bioproduction Of 2 High-Demand Chemicals
7/28/2016
A large variety of chemicals can be efficiently produced by microbial fermentation. They are used in a multitude of applications; for example, as biofuels, building blocks for polymers, food supplements, and ingredients for cosmetics. To be profitable, fermentation processes have to deliver the desired end product at a high yield and a high titer.
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Rapid Separation Of Basic Drug Compounds Under Alkaline pH Conditions On pH-Stable, Short .C18 Column
3/14/2013
Mobile phase pH is a powerful tool in methods development, particularly for separation of neutral forms of amines or other organic bases under alkaline conditions. By Derek Jensen and Mark Carrier, Hamilton Company
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Mammalian Virus Purification Using Ceramic Hydroxyapatite
Conventional techniques for mammalian virus purification produce variable quality, quantity, and significant loss of particle infectivity. We propose the chromatographic separation of viral particles of diverse sizes and from different families such as dengue, influenza, mouse hepatitis, adenovirus, poliovirus using CHT Ceramic Hydroxyapatite resin.
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Removal Of Host Cell Proteins During Monoclonal Antibody Purification
Protein A chromatography is a widely used and highly successful method of purifying recombinant monoclonal antibodies. However, since Protein A has high affinity for antibodies, harsh conditions such as low pH are often required to elute monoclonal antibodies from Protein A affinity columns. By 3M Purification Inc
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Discussion Of Vial Closures And Stoppering Force In Pilot Freeze-Dryers
To answer the often asked question "Can my system stopper a full tray of the vials and stoppers I have selected?,", it is important to be able to recognize the force needed for your particular container-closure system and to compare that to the stoppering pressure specification provided by the freeze dryer manufacturer. By Leslie Mather
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Rapid Screening Of A Cell-Based Assay For GLP-1 Receptor Using A Natural Product Library
8/9/2012
Here we demonstrate the detection of GLP-1R binding through the incorporation of a homogeneous cell-based binding assay using HTRF Tag-lite® technology. By Brad Larson and Peter Banks, BioTek Instruments, Inc. and Nicolas Pierre, Suzanne Graham, Jean-Luc Tardieu, and François Degorce, Cisbio US, Inc.
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Cultivation Of Human CAP Cells: Evaluation Of Scale-Down Capabilities
4/18/2016
The following application note illustrates how the DASbox Mini Bioreactor System combined with the BioBLU 0.3c single-use vessels supports bioprocess development in human cell culture.