Application Note: Use Of The Superpure 70 Automated System

Use of The Superpure 70 Automated System for Column Loadibility Study for Preparative Chiral Separation of Trans Stilbene Oxide (TSO) Anelly Vargas and Harbaksh Sidhu, Thar Technologies, Inc.

The SuperPure 70 System is an automated instrument developed by Thar Technologies for Supercritical Fluid Chromatography (SFC) and HPLC at preparative scale. The system is capable of separating chiral compounds and collecting pure enantiomers, with reliable, fast, and reproducible results.

Experimental:
We developed a method for the preparative chiral separation of Trans Stilbene Oxide (TSO) using Thar's SuperPure SFC70 System. Figure 2 shows the results of different loop volumes of 0.5 mL, 1.0 mL, 1.5 mL, and 2.0 mL and their injections under our defined optimal conditions. We used a concentration of TSO (98% purity) in MeOH of 54 mg/mL for 0.5 mL, 1.5 mL, and 2.0 mL. We also used 73% pure TSO with the cis impurity for the 1.0-mL loop at the same concentration.

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