TOYOPEARL® AF-rProtein A-650F

Protein A based affinity chromatography is the industry standard for the capture of monoclonal antibodies (MAbs) in downstream processing. Affinity media offer high selectivity thereby yielding a high purity of the target molecule in one step. The antibodies are captured at near neutral pH and eluted using acidic pH. The rising fermentation titres in the production of monoclonal antibodies drive the focus on developing resins that increase downstream capacity and efficiency for these processes.

Toyopearl AF-rProtein A-650F is an affinity resin designed for efficient and robust large-scale purification of monoclonal antibodies. A newly developed recombinant protein A ligand (E. coli) was linked to the well proven methacrylic polymer backbone of Toyopearl media. The ligand is derived from one of the IgG-binding domains of the staphylococcus aureus protein A. Its amino acid sequence was optimized in order to increase its stability towards alkaline solutions. Multipoint attachment of the ligand to the Toyopearl matrix further enhances chemical and thermal stability of the resin. In practice this pays off for a low level of protein A leaching and also for a high resistance to alkaline solutions employed in cleaning-inplace (CIP) procedures.