Download | April 1, 2008

Bulletin: Purification Of A Human Monoclonal IgM Antibody From Bioreactor Supernatant Using A Combination Of Cation And Anion Exchange

Few protocols are currently available for the purification of antibodies of IgM class. Here, we describe a novel procedure to purify a human IgM monoclonal antibody, SK- 1.45, with specificity for an adenocarcinoma associated antigen. SK-1.45 is produced by a hybridoma cell line in bioreactors using protein-free tissue culture media.

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