RNAimage Differential Display Kit
These kits feature the latest generation of isotopic mRNA Differential Display technology (U.S. Patents 5,262,311; 5,599,672; 5,665,547; 5,965,409 and pending patents). Each kit contains all the reagents needed for differential display except RNA samples, Taq DNA polymerase, and -[33P] dATP. The MessageClean® is highly recommended prior to differential display to remove trace amounts of DNA contamination from RNA samples.
The major improvements over the previous RNAmap technology are: (1) Optimized primer length ensures excellent reproducibility and maximized coverage of expressed genes. (2) One-base anchored primers minimize the redundancy of the displayed cDNA and simplify the reverse transcription reactions. (3) Attached HindIII site to the primers facilitates the manipulation of the cDNA after cloning.
References:
1. Liang, P. et al.: Differential display using one-base anchored oligo-dT primers. Nucleic Acids Res. 1994, 22:5763-5764.
2. Ikeda, S. et al.: An aquaporin-like gene required for the Brassica self-incompatibility response. Science. 1997, 276:1564-1566.
3. Taylor, G.A. et al: Identification of a novel GTPase, the inducibly expressed GTPase, that accumulates in response to Interferon [gamma]. J. Biol. Chem. 1996, 271:20399-20405.
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